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  • KD/KO Validated

KIF23 Polyclonal antibody

KIF23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28587-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

MKLP1, KNSL5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, U-87 MG cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28587-1-AP targets KIF23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF23 fusion protein Ag29346 相同性解析による交差性が予測される生物種
Full Name kinesin family member 23
Calculated molecular weight 110 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_138555
Gene Symbol KIF23
Gene ID (NCBI) 9493
RRIDAB_2881176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).

Protocols

Product Specific Protocols
WB protocol for KIF23 antibody 28587-1-APDownload protocol
IHC protocol for KIF23 antibody 28587-1-APDownload protocol
IF protocol for KIF23 antibody 28587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
humanWB,IHC

Front Cell Dev Biol

Identification of KIF23 as a Prognostic Biomarker Associated With Progression of Clear Cell Renal Cell Carcinoma.

Authors - Zonglong Wu
  • KD Validated
humanWB

Front Immunol

HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.

Authors - Mei-Ling Dong
humanWB

Toxicol Appl Pharmacol

Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway.

Authors - Xin He
  • KD Validated
WB

Cell Rep

Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.

Authors - Ana C Almeida
  • KD Validated
humanWB

Cell Discov

Packaged release and targeted delivery of cytokines by migrasomes in circulation

Authors - Haifeng Jiao
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