Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18833-1-AP targets KIF27 in IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 27 |
| Calculated molecular weight | 160 kDa |
| GenBank accession number | BC140788 |
| Gene Symbol | KIF27 |
| Gene ID (NCBI) | 55582 |
| RRID | AB_2878561 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF27 belongs to the kinesin-like protein family and it is a kinesin-4 motor with key roles in cytoskeletal function and ciliogenesis. KIF27 has a conserved function in microtubule organization which can influence microtubule dynamics. KIF27 is highly expressed in ciliated cells, such as olfactory sensory neurons, suggesting to be indispensable in the development and function of motile cilia. Unlike other family members, KIF27 is a slow motor due to its low phosphatase activity. (PMID: 29351996, 29450149, 21746835)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF27 antibody 18833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |