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  • KD/KO Validated

KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13105-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Kinesin-2, Kinesin like protein KIF2A, kinesin heavy chain member 2A, Kinesin 2, KIF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MDCK cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2A fusion protein Ag3748 相同性解析による交差性が予測される生物種
Full Name kinesin heavy chain member 2A
Calculated molecular weight 679 aa, 77 kDa
Observed molecular weight 80-100 kDa
GenBank accession numberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIF2A antibody 13105-1-APDownload protocol
IHC protocol for KIF2A antibody 13105-1-APDownload protocol
IF protocol for KIF2A antibody 13105-1-APDownload protocol
IP protocol for KIF2A antibody 13105-1-APDownload protocol
FC protocol for KIF2A antibody 13105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
  • KD Validated
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

Biomed Pharmacother

KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.

Authors - Xin Zhang
  • KD Validated
humanIF

Mol Biol Cell

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.

Authors - Hongdan Yang
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