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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using KIF2A Polyclonal antibody (27218-1-AP)

    WB analysis of Jurkat using 27218-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27218-1-AP (KIF2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KIF2A Polyclonal antibody (27218-1-AP)

    WB analysis of HeLa using 27218-1-AP

    WB result of KIF2A antibody (27218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF2A Polyclonal antibody (27218-1-AP)

    IHC staining of mouse brain using 27218-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27218-1-AP (KIF2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF2A Polyclonal antibody (27218-1-AP)

    IHC staining of mouse brain using 27218-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27218-1-AP (KIF2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KIF2A Polyclonal antibody (27218-1-AP)

    IF Staining of A549 using 27218-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (27218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  • Featured Product
  • KD/KO Validated

KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27218-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

27218-1-AP targets KIF2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2A fusion protein Ag25692 相同性解析による交差性が予測される生物種
Full Name kinesin heavy chain member 2A
Calculated molecular weight 679 aa, 77 kDa
Observed molecular weight 80 kDa~100 kDa
GenBank accession numberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2880805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).

Protocols

Product Specific Protocols
WB protocol for KIF2A antibody 27218-1-APDownload protocol
IHC protocol for KIF2A antibody 27218-1-APDownload protocol
IF protocol for KIF2A antibody 27218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Pathobiology

Cytomorphological disparities in invasive breast cancer cells following neoadjuvant endocrine therapy and chemotherapy

Authors - Hideko Hoshina
View Article