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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KIF2B Polyclonal antibody (13711-1-AP)

    WB analysis of mouse brain using 13711-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13711-1-AP (KIF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using KIF2B Polyclonal antibody (13711-1-AP)

    WB analysis of mouse testis using 13711-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13711-1-AP (KIF2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using KIF2B Polyclonal antibody (13711-1-AP)

    IP experiment of mouse testis using 13711-1-AP

    IP result of anti-KIF2B (IP:13711-1-AP, 4ug; Detection:13711-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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KIF2B Polyclonal antibody

KIF2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 13711-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

KIF2B, kinesin family member 2B, Kinesin like protein KIF2B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13711-1-AP targets KIF2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2B fusion protein Ag4648 相同性解析による交差性が予測される生物種
Full Name kinesin family member 2B
Calculated molecular weight 673 aa, 76 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC033802
Gene Symbol KIF2B
Gene ID (NCBI) 84643
RRIDAB_2131885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4N8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF2B belongs to the TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family and MCAK/KIF2 subfamily. KIF2B is evidently required for spindle assembly, chromosome movement and cytokinesis (PMID: 17538014). The molecular weight of KIF2B is 76 kDa. It plays an important role in chromosome congression (PMID: 23891108).

Protocols

Product Specific Protocols
WB protocol for KIF2B antibody 13711-1-APDownload protocol
IP protocol for KIF2B antibody 13711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols