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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF4A Polyclonal antibody

KIF4A Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14344-1-AP

Synonyms

KIF4 G1, KIF4, HSA271784, Chromosome-associated kinesin KIF4A, Chromokinesin-A

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIF4A Polyclonal antibody (14344-1-AP)

    IF Staining of HepG2 using 14344-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF4A antibody 14344-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14344-1-AP targets KIF4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF4A fusion protein Ag5682 相同性解析による交差性が予測される生物種
Full Name kinesin family member 4A
Calculated molecular weight 140 kDa
GenBank accession numberBC050548
Gene symbol KIF4A
Gene ID (NCBI) 24137
RRIDAB_2132200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for KIF4A antibody 14344-1-APDownload protocol
IF protocol for KIF4A antibody 14344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Clin Cancer Res

Targeting the KIF4A/AR axis to reverse endocrine therapy resistance in castration-resistant prostate cancer.

Authors - Ke Chen
View Article
humanWB,IHC

Anal Cell Pathol (Amst)

Aspirin Exerts Its Antitumor Effect in Esophageal Squamous Cell Carcinoma by Downregulating the Expression of ATAD2 and KIF4A

Authors - Man Zhang
View Article