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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF5A Polyclonal antibody (21186-1-AP)

    WB analysis using 21186-1-AP (same clone as 21186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21186-1-AP (KIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF5A Polyclonal antibody (21186-1-AP)

    IP experiment of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

    IP result of anti-KIF5A (IP:21186-1-AP, 4ug; Detection:21186-1-AP 1:2000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF5A Polyclonal antibody (21186-1-AP)

    IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF5A Polyclonal antibody (21186-1-AP)

    IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KIF5A Polyclonal antibody (21186-1-AP)

    IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KIF5A Polyclonal antibody (21186-1-AP)

    IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using KIF5A Polyclonal antibody (21186-1-AP)

    IF Staining of PC-12 using 21186-1-AP (same clone as 21186-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (21186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF5A Polyclonal antibody, PBS Only

KIF5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21186-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NKHC, Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21186-1-PBS targets KIF5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF5A fusion protein Ag15518 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 118-120 kDa
GenBank accession numberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
RRIDAB_10733125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12840
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.