• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF5A Monoclonal antibody

KIF5A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A7E3

Cat no : 67009-1-Ig

Synonyms

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KIF5A Monoclonal antibody (67009-1-Ig)

    WB analysis using 67009-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using KIF5A Monoclonal antibody (67009-1-Ig)

    WB analysis of pig brain using 67009-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using KIF5A Monoclonal antibody (67009-1-Ig)

    IF Staining of PC-12 using 67009-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (67009-1-Ig, Clone: 1A7E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inrat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67009-1-Ig targets KIF5A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIF5A fusion protein Ag15682 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC146670
Gene symbol KIF5A
Gene ID (NCBI) 3798
RRIDAB_2882326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Protocols

Product Specific Protocols
WB protocol for KIF5A antibody 67009-1-IgDownload protocol
IF protocol for KIF5A antibody 67009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols