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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00967-4

    Sandwich ELISA standard curve of MP00967-4

    Sandwich ELISA standard curve of MP00967-4, Human KIF5A Recombinant Matched Antibody Pair - PBS only. 84013-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15518. 84013-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00967-4

    Sample test of MP00967-4

    The mean KIF5A concentration was determined to be 10.30 ng/mL in mouse brain cell extract based on a 4.60 mg/mL extract load.

  • Sample test of MP00967-4

    Sample test of MP00967-4

    Serum of five individual healthy human donors was measured. The KIF5A concentration of detected samples was determined to be 1.00 ng/mL with a range of 0.50 - 1.35 ng/mL.

  • Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-3-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00967-3

    Cytometric bead array standard curve of MP00967-3

    Cytometric bead array standard curve of MP00967-3, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-3-PBS. Detection antibody: 84013-2-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84013-3-PBS

    Affinity and Kinetic Characterization of 84013-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 84013-3-PBS against Human KIF5A was performed. The affinity constant is 1.31 nM.

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KIF5A Recombinant antibody, PBS Only (Capture/Detector)

KIF5A Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84013-3-PBS
Clone No.241124F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84013-3-PBS targets KIF5A as part of a matched antibody pair:

MP00967-1: 84013-4-PBS capture and 84013-3-PBS detection (validated in Cytometric bead array)

MP00967-3: 84013-3-PBS capture and 84013-2-PBS detection (validated in Cytometric bead array)

MP00967-4: 84013-3-PBS capture and 84013-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIF5A fusion protein Ag15518 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
GenBank accession numberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12840
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.