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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF5A Recombinant antibody

KIF5A Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241124H5

Cat no : 84013-5-RR

Synonyms

Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using KIF5A Recombinant antibody (84013-5-RR)

    WB analysis of SH-SY5Y using 84013-5-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84013-5-RR (KIF5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using KIF5A Recombinant antibody (84013-5-RR)

    FC experiment of SH-SY5Y using 84013-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-5-RR, Clone:241124H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of K562 using KIF5A Recombinant antibody (84013-5-RR)

    FC experiment of K562 using 84013-5-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-5-RR, Clone:241124H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84013-5-RR

    Affinity and Kinetic Characterization of 84013-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-5-RR against Human KIF5A were performed. The affinity constant is 0.368 nM.

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Tested Applications

Positive WB detected inSH-SY5Y cells, U-87-MG cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inK562, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84013-5-RR is KIF5A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIF5A fusion protein Ag15518 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 118-120 kDa
GenBank accession numberBC146670
Gene symbol KIF5A
Gene ID (NCBI) 3798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Protocols

Product Specific Protocols
WB protocol for KIF5A antibody 84013-5-RRDownload protocol
FC protocol for KIF5A antibody 84013-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols