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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIF5B Recombinant antibody (82485-1-RR)

    WB analysis of HeLa using 82485-1-RR (same clone as 82485-1-PBS)

    WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KIF5B Recombinant antibody (82485-1-RR)

    WB analysis using 82485-1-RR (same clone as 82485-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using KIF5B Recombinant antibody (82485-1-RR)

    IHC staining of human ovarian cancer using 82485-1-RR (same clone as 82485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82485-1-RR (KIF5B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIF5B Recombinant antibody (82485-1-RR)

    IF Staining of HepG2 using 82485-1-RR (same clone as 82485-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIF5B antibody (82485-1-RR, Clone: 2B18 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF5B Recombinant antibody, PBS Only

KIF5B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82485-1-PBS
Clone No.2B18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82485-1-PBS targets KIF5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIF5B fusion protein Ag16250 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5B
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC126279
Gene Symbol KIF5B
Gene ID (NCBI) 3799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33176
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).