KIFAP3 Monoclonal antibody
KIFAP3 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
7C9F2
Cat no : 60266-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 60266-1-Ig is KIFAP3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KIFAP3 fusion protein Ag20809 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin-associated protein 3 |
| Calculated molecular weight | 792 aa, 91 kDa |
| Observed molecular weight | 91-100 kDa |
| GenBank accession number | BC028679 |
| Gene symbol | KIFAP3 |
| Gene ID (NCBI) | 22920 |
| RRID | AB_2881387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFAP3 antibody 60266-1-Ig | Download protocol |
| IHC protocol for KIFAP3 antibody 60266-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
