Validation Data Gallery
Tested Applications
| Positive WB detected in | rat testis tissue, HeLa cells, mouse testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human bowen disease, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
Product Information
20790-1-AP targets KIFC1 in WB, IHC, IF/ICC, ELISA, Peptide array applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KIFC1 fusion protein Ag14492 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member C1 |
| Calculated molecular weight | 673 aa, 74 kDa |
| Observed molecular weight | 55-70 kDa |
| GenBank accession number | BC000712 |
| Gene Symbol | KIFC1 |
| Gene ID (NCBI) | 3833 |
| RRID | AB_10755286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BW19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IHC protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IF protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Mechanism of spindle pole organization and instability in human oocytes.
| ||
Dev Cell Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds. | ||
Cell Mol Life Sci Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.
| ||
FASEB J Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1. | ||
Front Mol Biosci Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma. |