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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIR2DL3 Polyclonal antibody

KIR2DL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18966-1-AP

Synonyms

CD158b, CD158B2, GL183, KIR 023GB, KIR K7b, KIR K7c, KIR2DL3, KIRCL23, MHC class I NK cell receptor, NKAT, NKAT 2, NKAT2, NKAT2A, NKAT2B, p58, p58 NK receptor CL 6

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using KIR2DL3 Polyclonal antibody (18966-1-AP)

    WB analysis of human liver using 18966-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 18966-1-AP (KIR2DL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18966-1-AP targets KIR2DL3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIR2DL3 fusion protein Ag5163 相同性解析による交差性が予測される生物種
Full Name killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
Calculated molecular weight 341 aa, 38 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC032422
Gene symbol KIR2DL3
Gene ID (NCBI) 3804
RRIDAB_2130398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Killer cell immunoglobulin-like receptors (KIRs) are a diverse family of inhibitory and activating receptors expressed on NK cells and a subset of T cells (PMID: 11861603). These polymorphic receptors interact with specific motifs on HLA class I molecules, modulate NK cytolytic activity and are encoded by genes located on chromosome 19q13.4 (PMID: 17069649). KIR2DL3, also known as CD158B2 or NKAT2, is an inhibitory receptor that is specific for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7) (PMID: 10196125). It is a 341-amino acid transmembrane glycoprotein consisting of an extracellular region containing two C2-type Ig-like domains, a 19-amino acid hydrophobic transmembrane region, and a long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KIR2DL3 inhibits the cytolytic activity of NK cells thus preventing cell lysis (PMID: 7724594).

Protocols

Product Specific Protocols
WB protocol for KIR2DL3 antibody 18966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols