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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIRREL Polyclonal antibody

KIRREL Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 22232-1-AP

Synonyms

Nephrin-like protein 1, NEPH1, KIRREL1, Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIRREL Polyclonal antibody (22232-1-AP)

    WB analysis using 22232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22232-1-AP (KIRREL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using KIRREL Polyclonal antibody (22232-1-AP)

    IF Staining of mouse kidney using 22232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KIRREL antibody (22232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inC2C12 cells, mouse brain tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22232-1-AP targets KIRREL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIRREL fusion protein Ag17578 相同性解析による交差性が予測される生物種
Full Name kin of IRRE like (Drosophila)
Calculated molecular weight 757 aa, 84 kDa
Observed molecular weight72 kDa
GenBank accession numberBC109192
Gene symbol KIRREL
Gene ID (NCBI) 55243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kin of IRRE protein (KIRREL), also known as NEPH1, is a member of a podocin-binding protein family. KIRREL is a component of the podocyte slit diaphragm, which serves as the structural framework of the glomerular filtration barrier. KIRREL has three isoforms with molecular weights of 85 kDa, 83 kDa, and 72 kDa.

Protocols

Product Specific Protocols
WB protocol for KIRREL antibody 22232-1-APDownload protocol
IF protocol for KIRREL antibody 22232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols