Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 28100-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 28100-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 28100-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 28100-1-AP
IP result of anti-KL (IP:28100-1-AP, 4ug; Detection:28100-1-AP 1:300) with mouse kidney tissue lysate 4000 ug.
IHC staining of human kidney using 28100-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 28100-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28100-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28100-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28100-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KL Polyclonal antibody

KL Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 28100-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KL, klotho

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, rat kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human kidney tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 18 publications below
IHC	See 4 publications below
IF	See 2 publications below

Product Information

28100-1-AP targets KL in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KL fusion protein Ag27759 相同性解析による交差性が予測される生物種
Full Name	klotho
Calculated molecular weight	116 kDa
Observed molecular weight	62 kDa, 130 kDa
GenBank accession number	NM_004795
Gene Symbol	KL
Gene ID (NCBI)	9365
RRID	AB_2881060
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UEF7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for KL antibody 28100-1-AP	Download protocol
IHC protocol for KL antibody 28100-1-AP	Download protocol
IP protocol for KL antibody 28100-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo. Authors - Wen-Chih Liu View Article
mouse	WB	Ecotoxicol Environ Saf Effects of selenium-cadmium co-enriched Cardamine hupingshanensis on bone damage in mice Authors - Lin Zhang View Article
human	IHC	Cell Mol Life Sci α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway. Authors - Zewu Zhu View Article
rat	WB	Sci Rep PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats. Authors - Jing Li View Article
mouse	WB	Hum Cell Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis. Authors - Hao Meng View Article
rat	IHC	Toxics Low-Dose Propranolol Prevents Functional Decline in Catecholamine-Induced Acute Heart Failure in Rats. Authors - Cheng-Ken Tsai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 28100-1-AP

WB analysis of mouse kidney using 28100-1-AP

WB analysis of mouse kidney using 28100-1-AP

IP experiment of mouse kidney using 28100-1-AP

IHC staining of human kidney using 28100-1-AP

IHC staining of human kidney using 28100-1-AP

IHC staining of mouse brain using 28100-1-AP

IHC staining of mouse brain using 28100-1-AP

IHC staining of mouse brain using 28100-1-AP

KL Polyclonal antibody

KL Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.

Effects of selenium-cadmium co-enriched Cardamine hupingshanensis on bone damage in mice

α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.

PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.

Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis.

Low-Dose Propranolol Prevents Functional Decline in Catecholamine-Induced Acute Heart Failure in Rats.