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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLF1 Polyclonal antibody (18887-1-AP)

    WB analysis of HeLa using 18887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18887-1-AP (KLF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using KLF1 Polyclonal antibody (18887-1-AP)

    IF Staining of A431 using 18887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KLF1 antibody (18887-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KLF1 Polyclonal antibody

KLF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18887-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Krueppel-like factor 1, Krueppel like factor 1, Erythroid krueppel-like transcription factor, EKLF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18887-1-AP targets KLF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF1 fusion protein Ag13467 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 1 (erythroid)
Calculated molecular weight 362 aa, 38 kDa
GenBank accession numberBC033580
Gene Symbol KLF1
Gene ID (NCBI) 10661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLF1 antibody 18887-1-APDownload protocol
IF protocol for KLF1 antibody 18887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols