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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLF10 Polyclonal antibody

KLF10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29709-1-AP

Synonyms

EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLF10 Polyclonal antibody (29709-1-AP)

    WB analysis using 29709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KLF10 Polyclonal antibody (29709-1-AP)

    WB analysis using 29709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using KLF10 Polyclonal antibody (29709-1-AP)

    IHC staining of mouse pancreas using 29709-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using KLF10 Polyclonal antibody (29709-1-AP)

    IHC staining of mouse pancreas using 29709-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29709-1-AP targets KLF10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF10 fusion protein Ag31772 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 10
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight52-75 kDa
GenBank accession numberBC011538
Gene symbol KLF10
Gene ID (NCBI) 7071
RRIDAB_2918341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. KLF10 plays a role in the response to TGF beta (through the TGF beta/Smad signaling pathway) and estrogen. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer. KLF10 can be detected 51-52 kDa, 60-75 kDa (PMID: 21262377, PMID: 30026232).

Protocols

Product Specific Protocols
WB protocol for KLF10 antibody 29709-1-APDownload protocol
IHC protocol for KLF10 antibody 29709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols