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  • Featured Product
  • KD/KO Validated

KLF15 Monoclonal antibody, PBS Only

KLF15 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8G9

Cat no : 66185-1-PBS

Synonyms

DKFZp779M1320, KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using KLF15 Monoclonal antibody (66185-1-Ig)

    WB analysis of human skeletal muscle using 66185-1-Ig (same clone as 66185-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF15 Monoclonal antibody (66185-1-Ig)

    WB analysis using 66185-1-Ig (same clone as 66185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLF15 Monoclonal antibody (66185-1-Ig)

    IF Staining of HepG2 using 66185-1-Ig (same clone as 66185-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KLF15 antibody (66185-1-Ig, Clone: 2B8G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66185-1-PBS targets KLF15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen KLF15 fusion protein Ag4864 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 15
Calculated molecular weight 416 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC036733
Gene symbol KLF15
Gene ID (NCBI) 28999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).