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KD/KO Validated

KLF4 Polyclonal antibody

Name: KLF4 Polyclonal antibody
Brand: Proteintech
SKU: 11880-1-AP

KLF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Publications

133

Review Product

Cat no : 11880-1-AP

Synonyms

Krueppel-like factor 4, Krueppel like factor 4, GKLF, EZF

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11880-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11880-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 11880-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11880-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 11880-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 11880-1-AP
NCCIT cell were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11880-1-AP
IP result of anti-KLF4 (IP:11880-1-AP, 4ug; Detection:11880-1-AP 1:8000) with HeLa cells lysate 1320 ug.
IHC staining of mouse embryo using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsil using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsil using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 11880-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of human embronic stem cells using 11880-1-AP
Confocal immunofluorescent analysis of human embronic stem cells with 11880-1-AP at dilution of 1:50. The PE shows staining with 11880-1-AP/PE. The DAPI shows nuclear staining by DAPI.
FC experiment of HeLa using 11880-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KLF4 (11880-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 11880-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, A549 cells, HUVEC cells, NCCIT cells, HEK-293 cells, HT-29 cells, C2C12 cells, HT-1080 cells, HeLa cells, mouse liver tissue, mouse lung tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	human embronic stem cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 19 publications below
WB	See 105 publications below
IHC	See 20 publications below
IF	See 26 publications below
IP	See 5 publications below
FC	See 1 publications below
ChIP	See 12 publications below

Product Information

11880-1-AP targets KLF4 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLF4 fusion protein Ag2480 相同性解析による交差性が予測される生物種
Full Name	Kruppel-like factor 4 (gut)
Calculated molecular weight	504 aa, 54 kDa
Observed molecular weight	50-60 kDa
GenBank accession number	BC030811
Gene symbol	KLF4
Gene ID (NCBI)	9314
RRID	AB_10640807
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4 (PMID: 15561714), with MW range from 50 to 60 kDa,and major band is 55 kDa. KLF4 protein can be ubiquitin modified.

Protocols

Product Specific Protocols
WB protocol for KLF4 antibody 11880-1-AP	Download protocol
IHC protocol for KLF4 antibody 11880-1-AP	Download protocol
IP protocol for KLF4 antibody 11880-1-AP	Download protocol
FC protocol for KLF4 antibody 11880-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. Authors - Buqing Ye View Article
human	WB,IHC	Nat Commun LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells. Authors - Pingping Zhu View Article
human	WB,IHC	J Hematol Oncol A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties. Authors - Shuiqing Liu View Article
human	IF	Mater Today Bio Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles. Authors - Mei-Hwa Lee View Article
mouse	WB	EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. Authors - Lihua Wang View Article
mouse	IF,WB	J Neuroinflammation The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke Authors - Cong Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLF4 Polyclonal antibody

KLF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 11880-1-AP

WB analysis using 11880-1-AP

WB analysis using 11880-1-AP

WB analysis using 11880-1-AP

WB analysis using 11880-1-AP

WB analysis of NCCIT using 11880-1-AP

IP experiment of HeLa using 11880-1-AP

IHC staining of mouse embryo using 11880-1-AP

IHC staining of mouse embryo using 11880-1-AP

IHC staining of human tonsil using 11880-1-AP

IHC staining of human tonsil using 11880-1-AP

IHC staining of human spleen using 11880-1-AP

IHC staining of human spleen using 11880-1-AP

IHC staining of human colon using 11880-1-AP

IHC staining of human colon using 11880-1-AP

IF Staining of human embronic stem cells using 11880-1-AP

FC experiment of HeLa using 11880-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.

Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles.

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke

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