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KD/KO Validated

KLF5 Polyclonal antibody

Name: KLF5 Polyclonal antibody
Brand: Proteintech
SKU: 21017-1-AP
Rating: 3.00 (1 reviews)

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 21017-1-AP

Synonyms

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 21017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 21017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 21017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 21017-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 21017-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21017-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 21017-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21017-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21017-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21017-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 21017-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21017-1-AP
IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of mouse embryo using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 21017-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 21017-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (21017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 21017-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue, mouse embryo tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 14 publications below
WB	See 38 publications below
IHC	See 12 publications below
IF	See 11 publications below
IP	See 1 publications below
CoIP	See 4 publications below
ChIP	See 6 publications below

Product Information

21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLF5 fusion protein Ag15267 相同性解析による交差性が予測される生物種
Full Name	Kruppel-like factor 5 (intestinal)
Calculated molecular weight	457 aa, 51 kDa
Observed molecular weight	51-55 kDa
GenBank accession number	BC042131
Gene symbol	KLF5
Gene ID (NCBI)	688
RRID	AB_10696447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

Protocols

Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-AP	Download protocol
IHC protocol for KLF5 antibody 21017-1-AP	Download protocol
IF protocol for KLF5 antibody 21017-1-AP	Download protocol
IP protocol for KLF5 antibody 21017-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection Authors - Jiakai Hou View Article
human	WB	Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. Authors - Shunrong Ji View Article
human	WB,IHC	Oncogene KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma Authors - Zhenyu Yang View Article
human	WB	Cell Death Dis DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma. Authors - Rong-Jie Fu KD Validated View Article
human	WB,IHC	Aging (Albany NY) Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer. Authors - Yilong Li KD Validated View Article
human	WB	Oncotarget Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells. Authors - Xiaolong Wei KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Sarah-Eve (Verified Customer) (03-01-2023)

Works okay on pvdf membrane, blocking with skim milk. A lot of bands.

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: Human pulmonary smooth muscle cells

KLF5 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary smooth muscle cells (Cat no:21017-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 21017-1-AP

WB analysis using 21017-1-AP

WB analysis using 21017-1-AP

WB analysis using 21017-1-AP

WB analysis of human testis using 21017-1-AP

WB analysis of PC-3 using 21017-1-AP

WB analysis of HT-1080 using 21017-1-AP

WB analysis of mouse small intestine using 21017-1-AP

WB analysis of A549 using 21017-1-AP

WB analysis of A549 using 21017-1-AP

WB analysis of mouse colon using 21017-1-AP

IP experiment of HeLa using 21017-1-AP

IHC staining of mouse embryo using 21017-1-AP

IHC staining of human bowen disease using 21017-1-AP

IHC staining of human bowen disease using 21017-1-AP

IHC staining of human colon cancer using 21017-1-AP

IHC staining of human colon cancer using 21017-1-AP

IHC staining of mouse embryo using 21017-1-AP

IF Staining of HeLa using 21017-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Reviews