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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLF5 Recombinant antibody

KLF5 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230509G7

Cat no : 83205-4-RR

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLF5 Recombinant antibody (83205-4-RR)

    IF Staining of HeLa using 83205-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using KLF5 Recombinant antibody (83205-4-RR)

    FC experiment of A549 using 83205-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HT-29 cells using KLF5 Recombinant antibody (83205-4-RR)

    FC experiment of HT-29 using 83205-4-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83205-4-RR

    Affinity and Kinetic Characterization of 83205-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83205-4-RR is KLF5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KLF5 fusion protein Ag15267 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC042131
Gene symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

Protocols

Product Specific Protocols
IF protocol for KLF5 antibody 83205-4-RRDownload protocol
FC protocol for KLF5 antibody 83205-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols