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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLF9 Recombinant antibody (83183-4-RR)

    WB analysis using 83183-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using KLF9 Recombinant antibody (83183-4-RR)

    FC experiment of A549 using 83183-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83183-4-RR

    Affinity and Kinetic Characterization of 83183-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

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KLF9 Recombinant antibody

KLF9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83183-4-RR
Clone No.230436H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83183-4-RR targets KLF9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KLF9 fusion protein Ag34411 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 9
Calculated molecular weight27 kDa
Observed molecular weight27-35 kDa
GenBank accession numberBC074880
Gene Symbol KLF9
Gene ID (NCBI) 687
RRIDAB_3670873
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13886
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Krüppel-like factor 9 (KLF9) was a member of SP/KLF family of DNA-binding transcriptional regulators featured by a highly homologous C-terminal three C2H2-type zinc finger DNA-binding domains. KLF9 has been previously reported to be downregulated in various types of human malignancy and serves as a tumour suppressor at tumour onset and development, affecting cell proliferation, apoptosis, metastasis and tumorigenicity (PMID: 32855660).

Protocols

Product Specific Protocols
WB protocol for KLF9 antibody 83183-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols