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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL11 Polyclonal antibody

KLHL11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17117-1-AP

Synonyms

kelch like 11 (Drosophila), Kelch like protein 11, KLHL11

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using KLHL11 Polyclonal antibody (17117-1-AP)

    WB analysis of COLO 320 using 17117-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17117-1-AP (KLHL11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KLHL11 Polyclonal antibody (17117-1-AP)

    WB analysis of MCF-7 using 17117-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17117-1-AP (KLHL11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCOLO 320 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17117-1-AP targets KLHL11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL11 fusion protein Ag10805 相同性解析による交差性が予測される生物種
Full Name kelch-like 11 (Drosophila)
Calculated molecular weight 708 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC034470
Gene symbol KLHL11
Gene ID (NCBI) 55175
RRIDAB_2130733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLHL11 is a component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating ubiquitination of target proteins. The presence of antibodies directed against KLHL11 has been identified as biomarker of a paraneoplastic neurological syndrome. The downregulation of KLHL11 may contribute to the disease progression, owing to its putative function in protein quality control. The calculated molecular weight of KLHL11 is 80 kDa (PMID: 34312900).

Protocols

Product Specific Protocols
WB protocol for KLHL11 antibody 17117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols