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  • Featured Product
  • KD/KO Validated

KLHL12 Polyclonal antibody

KLHL12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14883-1-AP

Synonyms

hDKIR, DKIR homolog, DKIR, CUL3-interacting protein 1, CUL3 interacting protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    WB analysis of PC-3 using 14883-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    WB analysis of PC-3 using 14883-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL12 Polyclonal antibody (14883-1-AP)

    IHC staining of human testis using 14883-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL12 Polyclonal antibody (14883-1-AP)

    IHC staining of human testis using 14883-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    IF Staining of PC-3 using 14883-1-AP

    Immunofluorescent analysis of PC-3 cells using 14883-1-AP (KLHL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14883-1-AP targets KLHL12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL12 fusion protein Ag6658 相同性解析による交差性が予測される生物種
Full Name kelch-like 12 (Drosophila)
Calculated molecular weight 63 kDa
Observed molecular weight 53-56 kDa, 63-67 kDa
GenBank accession numberBC004175
Gene symbol KLHL12
Gene ID (NCBI) 59349
RRIDAB_10644282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL12 antibody 14883-1-APDownload protocol
IHC protocol for KLHL12 antibody 14883-1-APDownload protocol
IF protocol for KLHL12 antibody 14883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Authors - David Akopian
View Article
WB, IF

Arterioscler Thromb Vasc Biol

A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report.

Authors - Chutikarn Butkinaree
  • KD Validated
View Article