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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    WB analysis of PC-3 using 16693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    WB analysis of HepG2 using 16693-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    WB analysis of PC-3 using 16693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    WB analysis of MCF-7 using 16693-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    WB analysis of HepG2 using 16693-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLHL14 Polyclonal antibody (16693-1-AP)

    IF Staining of HepG2 using 16693-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14 antibody (16693-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16693-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16693-1-AP targets KLHL14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL14 fusion protein Ag10065 相同性解析による交差性が予測される生物種
Full Name kelch-like 14 (Drosophila)
Calculated molecular weight 44 kDa, 71 kDa
Observed molecular weight35 kDa, 70 kDa
GenBank accession numberBC021267
Gene Symbol KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.

Protocols

Product Specific Protocols
WB protocol for KLHL14 antibody 16693-1-APDownload protocol
IF protocol for KLHL14 antibody 16693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols