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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29253-1-AP

Synonyms

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL14 Polyclonal antibody (29253-1-AP)

    WB analysis using 29253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29253-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KLHL14 Polyclonal antibody (29253-1-AP)

    WB analysis of mouse brain using 29253-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 29253-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLHL14 Polyclonal antibody (29253-1-AP)

    IF Staining of HepG2 using 29253-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14 antibody (29253-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse kidney tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29253-1-AP targets KLHL14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL14 fusion protein Ag29188 相同性解析による交差性が予測される生物種
Full Name kelch-like 14 (Drosophila)
Calculated molecular weight 44 kDa, 71 kDa
Observed molecular weight43 kDa, 70 kDa
GenBank accession numberBC021267
Gene symbol KLHL14
Gene ID (NCBI) 57565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.

Protocols

Product Specific Protocols
WB protocol for KLHL14 antibody 29253-1-APDownload protocol
IF protocol for KLHL14 antibody 29253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols