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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHL15 Polyclonal antibody (25066-1-AP)

    WB analysis of HeLa using 25066-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KLHL15 Polyclonal antibody (25066-1-AP)

    WB analysis of HEK-293 using 25066-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human prostate cancer using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human prostate cancer using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human brain using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human brain using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL15 Polyclonal antibody (25066-1-AP)

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 10x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KLHL15 Polyclonal antibody

KLHL15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25066-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25066-1-AP targets KLHL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL15 fusion protein Ag16878 相同性解析による交差性が予測される生物種
Full Name kelch-like 15 (Drosophila)
Calculated molecular weight 604 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC109058
Gene Symbol KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL15 antibody 25066-1-APDownload protocol
IHC protocol for KLHL15 antibody 25066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation.

Authors - Chuanchao Zhang
View Article
humanWB

Sci Adv

VGLL3 modulates chemosensitivity through promoting DNA double-strand break repair

Authors - Wei Wu
  • KD Validated
View Article