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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL25 Polyclonal antibody

KLHL25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27482-1-AP

Synonyms

ENC 2, ENC2, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL25 Polyclonal antibody (27482-1-AP)

    WB analysis using 27482-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27482-1-AP (KLHL25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLHL25 Polyclonal antibody (27482-1-AP)

    IHC staining of mouse brain using 27482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLHL25 Polyclonal antibody (27482-1-AP)

    IHC staining of mouse brain using 27482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KLHL25 Polyclonal antibody (27482-1-AP)

    IF Staining of A549 using 27482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KLHL25 antibody (27482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27482-1-AP targets KLHL25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL25 fusion protein Ag25765 相同性解析による交差性が予測される生物種
Full Name kelch-like 25 (Drosophila)
Observed molecular weight66 kDa
GenBank accession numberBC028100
Gene symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2880883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL25 antibody 27482-1-APDownload protocol
IHC protocol for KLHL25 antibody 27482-1-APDownload protocol
IF protocol for KLHL25 antibody 27482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols