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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL28 Polyclonal antibody

KLHL28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25224-1-AP

Synonyms

BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using KLHL28 Polyclonal antibody (25224-1-AP)

    WB analysis of Jurkat using 25224-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    WB analysis of mouse brain using 25224-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human colon using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using KLHL28 Polyclonal antibody (25224-1-AP)

    IHC staining of human colon using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25224-1-AP targets KLHL28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL28 fusion protein Ag18665 相同性解析による交差性が予測される生物種
Full Name kelch-like 28 (Drosophila)
Calculated molecular weight 571 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC121009
Gene symbol KLHL28
Gene ID (NCBI) 54813
RRIDAB_2879972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLHL28 antibody 25224-1-APDownload protocol
IHC protocol for KLHL28 antibody 25224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols