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KLHL3 Polyclonal antibody

Name: KLHL3 Polyclonal antibody
Brand: Proteintech
SKU: 16951-1-AP

KLHL3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16951-1-AP

Synonyms

KIAA1129, Kelch-like protein 3, Kelch like protein 3

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of HepG2 using 16951-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16951-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16951-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16951-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16951-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 16951-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 16951-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16951-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 16951-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16951-1-AP
IP result of anti-KLHL3 (IP:16951-1-AP, 4ug; Detection:16951-1-AP 1:700) with HepG2 cells lysate 2800ug.
IHC staining of human liver using 16951-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human tonsillitis using 16951-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16951-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human tonsillitis using 16951-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 16951-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.

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Cat No. 16951-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, Jurkat cells, rat liver tissue, human liver tissue, mouse thymus tissue, HL-60 cells, mouse pancreas tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human tonsillitis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

16951-1-AP targets KLHL3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLHL3 fusion protein Ag10580 相同性解析による交差性が予測される生物種
Full Name	kelch-like 3 (Drosophila)
Calculated molecular weight	65 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC034035
Gene symbol	KLHL3
Gene ID (NCBI)	26249
RRID	AB_2130861
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL3 antibody 16951-1-AP	Download protocol
IHC protocol for KLHL3 antibody 16951-1-AP	Download protocol
IP protocol for KLHL3 antibody 16951-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nat Genet KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron. Authors - Louis-Dit-Picard Hélène H View Article
human	WB,IHC	Cell Death Differ A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA. Authors - Xiaoyu Qi View Article
mouse	WB,IF	J Am Soc Nephrol Combined Kelch-Like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice. Authors - Yujiro Maeoka View Article
mouse	WB	J Am Soc Nephrol Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus. Authors - Moko Zeniya View Article
mouse	WB	J Am Soc Nephrol Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway. Authors - Ryan J Cornelius View Article
mouse	IF	Hum Mol Genet Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice. Authors - Koichiro Susa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL3 Polyclonal antibody

KLHL3 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of HepG2 using 16951-1-AP

WB analysis of mouse liver using 16951-1-AP

WB analysis of Jurkat using 16951-1-AP

WB analysis of HepG2 using 16951-1-AP

WB analysis of human liver using 16951-1-AP

WB analysis of mouse thymus using 16951-1-AP

WB analysis of HL-60 using 16951-1-AP

WB analysis of rat liver using 16951-1-AP

WB analysis of mouse pancreas using 16951-1-AP

IP experiment of HepG2 using 16951-1-AP

IHC staining of human liver using 16951-1-AP

IHC staining of human tonsillitis using 16951-1-AP

IHC staining of human liver using 16951-1-AP

IHC staining of human tonsillitis using 16951-1-AP

IHC staining of human tonsillitis using 16951-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Combined Kelch-Like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice.

Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.

Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.

Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.