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KLHL31 Polyclonal antibody
KLHL31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 24781-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse embryo tissue, Jurkat cells |
Positive IHC detected in | mouse skeletal muscle tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
24781-1-AP targets KLHL31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KLHL31 fusion protein Ag20462 相同性解析による交差性が予測される生物種 |
Full Name | kelch-like 31 (Drosophila) |
Calculated molecular weight | 634 aa, 70 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | BC137267 |
Gene symbol | KLHL31 |
Gene ID (NCBI) | 401265 |
RRID | AB_2879718 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KLHL31 also named as BKLHD6, KBTBD1,or Kelch like protein 31 is a 634 amino acid protein, which contains one 1 BACK domain, one BTB domain and six Kelch repeats. KLHL31 as a transcription repressor in MAPK/JNK signaling pathway to regulate cellular functions. KLHL31 is strongly expressed in skeletal muscle and weakly in heart.
Protocols
Product Specific Protocols | |
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WB protocol for KLHL31 antibody 24781-1-AP | Download protocol |
IHC protocol for KLHL31 antibody 24781-1-AP | Download protocol |
IF protocol for KLHL31 antibody 24781-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |