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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL35 Polyclonal antibody

KLHL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21199-1-AP

Synonyms

26597, kelch like 35 (Drosophila), Kelch like protein 35, KLHL35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL35 Polyclonal antibody (21199-1-AP)

    WB analysis using 21199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21199-1-AP (KLHL35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL35 Polyclonal antibody (21199-1-AP)

    IHC staining of human heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21199-1-AP (KLHL35 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL35 Polyclonal antibody (21199-1-AP)

    IHC staining of human heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21199-1-AP (KLHL35 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KLHL35 Polyclonal antibody (21199-1-AP)

    IHC staining of mouse heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21199-1-AP (KLHL35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KLHL35 Polyclonal antibody (21199-1-AP)

    IHC staining of mouse heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21199-1-AP (KLHL35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLHL35 Polyclonal antibody (21199-1-AP)

    IF Staining of HeLa using 21199-1-AP

    Immunofluorescent analysis of Hela cells, using KLHL35 antibody 21199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21199-1-AP is KLHL35 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL35 fusion protein Ag15558 相同性解析による交差性が予測される生物種
Full Name kelch-like 35 (Drosophila)
Calculated molecular weight 363 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC042952
Gene symbol KLHL35
Gene ID (NCBI) 283212
RRIDAB_10693685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL35 antibody 21199-1-APDownload protocol
IHC protocol for KLHL35 antibody 21199-1-APDownload protocol
IF protocol for KLHL35 antibody 21199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols