Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
10812-1-AP targets Kallikrein 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Kallikrein 2 fusion protein Ag1077 相同性解析による交差性が予測される生物種 |
| Full Name | kallikrein-related peptidase 2 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 29 kda |
| GenBank accession number | BC005196 |
| Gene Symbol | KLK2 |
| Gene ID (NCBI) | 3817 |
| RRID | AB_2134232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KLK2(Kallikrein-2) is also named as hGK-1 and belongs to the Kallikrein subfamily. It plays a role in prostate cancer susceptibility and this role may be realized at least in part by the induction of increases in its production. It also may mediate tumor growth and invasion by participating in proteolytic cascades. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 29 kDa, 25 kDa, 18 kDa and 17 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Kallikrein 2 antibody 10812-1-AP | Download protocol |
| IHC protocol for Kallikrein 2 antibody 10812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Photochem Photobiol B Inhibition of hypoxia-inducible factor 1 by acriflavine renders glioblastoma sensitive for photodynamic therapy | ||
Cancer Med Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation |