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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLK3/PSA Monoclonal antibody, PBS Only

KLK3/PSA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A1E2

Cat no : 60338-1-PBS

Synonyms

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    WB analysis using 60338-1-Ig (same clone as 60338-1-PBS)

    PC-3(PSA-), DU 145(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Monoclonal antibody (60338-1-Ig)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60338-1-PBS targets KLK3/PSA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLK3/PSA fusion protein Ag1060 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 3
Calculated molecular weight 29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC005307
Gene symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.