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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLK3/PSA Recombinant antibody

KLK3/PSA Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241263F8

Cat no : 84059-5-RR

Synonyms

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of LNCaP cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    WB analysis of LNCaP using 84059-5-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-5-RR (KLK3/PSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    IF Staining of LNCaP using 84059-5-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using KLK3/PSA antibody (84059-5-RR, Clone: 241263F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    FC experiment of HeLa using 84059-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug KLK3/PSA Recombinant antibody (84059-5-RR, Clone:241263F8) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84059-5-RR

    Affinity and Kinetic Characterization of 84059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84059-5-RR against Human KLK3/PSA were performed. The affinity constant is 149 nM.

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Tested Applications

Positive WB detected inLNCaP cells
Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84059-5-RR is KLK3/PSA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 3
Calculated molecular weight29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberNM_001648.2
Gene symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLK3/PSA antibody 84059-5-RRDownload protocol
IF protocol for KLK3/PSA antibody 84059-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols