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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLK4 Polyclonal antibody

KLK4 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26547-1-AP

Synonyms

ARM1, EMSP, EMSP1, Kallikrein 4, Kallikrein like protein 1, kallikrein related peptidase 4, KLK L1, KLK4, PROSTASE, PRSS17, PSTS, Serine protease 17

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of DU 145 cells using KLK4 Polyclonal antibody (26547-1-AP)

    IP experiment of DU 145 using 26547-1-AP

    IP result of anti-KLK4 (IP:26547-1-AP, 4ug; Detection:26547-1-AP 1:300) with DU 145 cells lysate 2200ug.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using KLK4 Polyclonal antibody (26547-1-AP)

    IHC staining of human prostate hyperplasia using 26547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using KLK4 Polyclonal antibody (26547-1-AP)

    IHC staining of human prostate hyperplasia using 26547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive IP detected inDU 145 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26547-1-AP is KLK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLK4 fusion protein Ag24240 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 4
Calculated molecular weight 254 aa, 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC069325
Gene symbol KLK4
Gene ID (NCBI) 9622
RRIDAB_2880548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for KLK4 antibody 26547-1-APDownload protocol
IP protocol for KLK4 antibody 26547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Am J Pathol

Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis

Authors - Jingwen Shi
View Article