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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kallikrein 8 Polyclonal antibody

Kallikrein 8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14232-1-AP

Synonyms

KLK8, KLK 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Kallikrein 8 Polyclonal antibody (14232-1-AP)

    WB analysis of HeLa using 14232-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14232-1-AP (Kallikrein 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Kallikrein 8 Polyclonal antibody (14232-1-AP)

    IHC staining of human ovary cancer using 14232-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14232-1-AP (Kallikrein 8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14232-1-AP targets Kallikrein 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kallikrein 8 fusion protein Ag5472 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 8
Calculated molecular weight 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC040887
Gene symbol KLK8
Gene ID (NCBI) 11202
RRIDAB_2134643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
IHC protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Authors - Tongmei Zhang
View Article