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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NKG2A Polyclonal antibody

NKG2A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10935-1-AP

Synonyms

KLRC1, NKG2-A/B-activating NK receptor, NKG2, NK cell receptor A, CD159A

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using NKG2A Polyclonal antibody (10935-1-AP)

    WB analysis of human liver using 10935-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10935-1-AP (NKG2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NKG2A Polyclonal antibody (10935-1-AP)

    IHC staining of human tonsillitis using 10935-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NKG2A Polyclonal antibody (10935-1-AP)

    IHC staining of human tonsillitis using 10935-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using NKG2A Polyclonal antibody (10935-1-AP)

    IF Staining of human tonsillitis using 10935-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NKG2A antibody (10935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10935-1-AP targets NKG2A in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKG2A fusion protein Ag1376 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily C, member 1
Calculated molecular weight 26 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC012550
Gene symbol NKG2A
Gene ID (NCBI) 3821
RRIDAB_2249683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLRC1, also known as NKG2A (NKG2) and CD159A, is a single-pass type II membrane protein with extracellular C-type lectin domain. It belongs to the natural killer cell lectin-like receptor family, which is a group of transmembrane proteins preferentially expressed in NK cells. KLRC1 can form a heterodimer with CD94. The NKG2A/CD94 complex is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells.

Protocols

Product Specific Protocols
WB protocol for NKG2A antibody 10935-1-APDownload protocol
IHC protocol for NKG2A antibody 10935-1-APDownload protocol
IF protocol for NKG2A antibody 10935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS One

Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis.

Authors - Yi Li
View Article