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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLRF1 Polyclonal antibody

KLRF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21510-1-AP

Synonyms

Activating coreceptor NKp80, CLEC5C, KLRF1, Lectin like receptor F1, ML, NKp80

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using KLRF1 Polyclonal antibody (21510-1-AP)

    WB analysis of human spleen using 21510-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 21510-1-AP (KLRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using KLRF1 Polyclonal antibody (21510-1-AP)

    IHC staining of human tonsillitis using 21510-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using KLRF1 Polyclonal antibody (21510-1-AP)

    IHC staining of human tonsillitis using 21510-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21510-1-AP is KLRF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLRF1 fusion protein Ag16008 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily F, member 1
Calculated molecular weight 231 aa, 27 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC098257
Gene symbol KLRF1
Gene ID (NCBI) 51348
RRIDAB_2878873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLRF1 antibody 21510-1-APDownload protocol
IHC protocol for KLRF1 antibody 21510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols