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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KMT2C Recombinant antibody, PBS Only

KMT2C Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240542A2

Cat no : 83595-4-PBS

Synonyms

MLL3, Lysine N methyltransferase 2C, KIAA1506, Homologous to ALR protein, HALR

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KMT2C Recombinant antibody (83595-4-RR)

    IF Staining of MCF-7 using 83595-4-RR (same clone as 83595-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMT2C antibody (83595-4-RR, Clone: 240542A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (green). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using KMT2C Recombinant antibody (83595-4-RR)

    FC experiment of U2OS using 83595-4-RR (same clone as 83595-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug KMT2C Recombinant antibody (83595-4-RR, Clone:240542A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83595-4-RR

    Affinity and Kinetic Characterization of 83595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83595-4-RR against Human KMT2C were performed. The affinity constant is 2.67 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83595-4-PBS is KMT2C Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KMT2C fusion protein Ag28875 相同性解析による交差性が予測される生物種
Full Name myeloid/lymphoid or mixed-lineage leukemia 3
Calculated molecular weight 541 kDa
GenBank accession numberNM_170606
Gene symbol KMT2C
Gene ID (NCBI) 58508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.