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KD/KO Validated

KMT2D Polyclonal antibody

Name: KMT2D Polyclonal antibody
Brand: Proteintech
SKU: 27266-1-AP

KMT2D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27266-1-AP

Synonyms

MLL2, KMT2D/MLL2

Validation Data Gallery

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AllWBIHC

WB analysis using 27266-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27266-1-AP (KMT2D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 27266-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27266-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 27266-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 27266-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 27266-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 27266-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human lymphoma tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 6 publications below
IF	See 3 publications below
ChIP	See 3 publications below

Product Information

27266-1-AP targets KMT2D in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KMT2D fusion protein Ag26195 相同性解析による交差性が予測される生物種
Full Name	myeloid/lymphoid or mixed-lineage leukemia 2
Calculated molecular weight	593 kDa
Observed molecular weight	593 kDa
GenBank accession number	NM_003482
Gene symbol	KMT2D/MLL2
Gene ID (NCBI)	8085
RRID	AB_2880823
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KMT2D (a COMPASS/ Set1 family member; also known as MLL4, ALR, and MLL2) is the biggest H3K4 methyltransferase among the most frequently mutated genes in many different types of cancer (PMID: 34194626). The enzymatic function of KMT2D depends on a cluster of C-terminal conserved domains, including a PHD domain, two FY-rich motifs (FYRC and FYRN) and a catalytic SET domain. KMT2D has two frequently occurring genomic alterations: truncation and missense mutations. Loss of KMT2D is a bona fide tumor suppressor gene in FL and DLBCL. Kmt2d perturbed the expression of genes that sustain proliferation and survival, and the KMT2D protein directly binds and associates with an active chromatin conformation in negative modulators of the BCR and lymphocyte migration pathways, which in turn could affect B cell responses to antigen(PMID: 26366712).

Protocols

Product Specific Protocols
WB protocol for KMT2D antibody 27266-1-AP	Download protocol
IHC protocol for KMT2D antibody 27266-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Immunol Res Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis. Authors - Xuanwen Bao View Article
human	IHC	Oncogene Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3. Authors - Xuejiao Leng View Article
human	IHC,IF	Cell Biosci Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma. Authors - Xinmiao Wang View Article
human	IHC	Thorac Cancer RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules. Authors - Ning Jiang View Article
human	WB,IHC	Transl Cancer Res Histone methyltransferase KMT2D mediated lipid metabolism via peroxisome proliferator-activated receptor gamma in prostate cancer Authors - Qiliang Zhai View Article
	ChIP	Cell Death Discov Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment. Authors - Qianshuo Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KMT2D Polyclonal antibody

KMT2D Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27266-1-AP

IHC staining of human breast cancer using 27266-1-AP

IHC staining of human breast cancer using 27266-1-AP

IHC staining of human colon cancer using 27266-1-AP

IHC staining of human lymphoma using 27266-1-AP

IHC staining of human lymphoma using 27266-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis.

Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3.

Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma.

RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.

Histone methyltransferase KMT2D mediated lipid metabolism via peroxisome proliferator-activated receptor gamma in prostate cancer

Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment.