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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KNL1 Polyclonal antibody

KNL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28695-1-AP

Synonyms

CASC5, D40, CT29, Cancer/testis antigen 29, Blinkin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KNL1 Polyclonal antibody (28695-1-AP)

    WB analysis using 28695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28695-1-AP (KNL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KNL1 Polyclonal antibody (28695-1-AP)

    IF Staining of U2OS using 28695-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KNL1 antibody (28695-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28695-1-AP targets KNL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KNL1 fusion protein Ag29881 相同性解析による交差性が予測される生物種
Full Name cancer susceptibility candidate 5
Calculated molecular weight 265 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_170589
Gene symbol CASC5/KNL1
Gene ID (NCBI) 57082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KNL1 is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. KNL1 functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues.

Protocols

Product Specific Protocols
WB protocol for KNL1 antibody 28695-1-APDownload protocol
IF protocol for KNL1 antibody 28695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols