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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KNTC1 Polyclonal antibody (24694-1-AP)

    WB analysis using 24694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24694-1-AP (KNTC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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KNTC1 Polyclonal antibody

KNTC1 Polyclonal Antibody for WB, ELISA
Cat No. 24694-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Kinetochore-associated protein 1, Kinetochore associated protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, NCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24694-1-AP targets KNTC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KNTC1 fusion protein Ag20268 相同性解析による交差性が予測される生物種
Full Name kinetochore associated 1
Calculated molecular weight251 kDa
Observed molecular weight210-230 kDa
GenBank accession numberBC150278
Gene Symbol KNTC1
Gene ID (NCBI) 9735
RRIDAB_3669445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KNTC1 is a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Northern blot analysis revealed low-to-moderate expression in all tissues tested, with highest expression in testis.

Protocols

Product Specific Protocols
WB protocol for KNTC1 antibody 24694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols