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Importin Alpha 5 Polyclonal antibody

Name: Importin Alpha 5 Polyclonal antibody
Brand: Proteintech
SKU: 18137-1-AP
Rating: 4.00 (1 reviews)

Importin Alpha 5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 18137-1-AP

Synonyms

IMA5, KPNA1, IPOA5, Impα5, Importin subunit alpha-5, N-terminally processed

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 18137-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18137-1-AP (importin α5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18137-1-AP
WB result of Importin Alpha 5 antibody (18137-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HepG2 cells.
IP experiment of HEK-293 using 18137-1-AP
IP result of anti-Importin Alpha 5 (IP:18137-1-AP, 4ug; Detection:18137-1-AP 1:1000) with HEK-293 cells lysate 1480ug.
IHC staining of human testis using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 18137-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 18137-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Importin Alpha 5 antibody (18137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 18137-1-AP
Immunofluorescent analysis of MCF-7 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 18137-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human testis tissue, human kidney tissue, human placenta tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 25 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

18137-1-AP targets Importin Alpha 5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Importin Alpha 5 fusion protein Ag12751 相同性解析による交差性が予測される生物種
Full Name	karyopherin alpha 1 (importin alpha 5)
Calculated molecular weight	60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC002374
Gene symbol	Importin Alpha 5
Gene ID (NCBI)	3836
RRID	AB_2133553
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

Protocols

Product Specific Protocols
WB protocol for Importin Alpha 5 antibody 18137-1-AP	Download protocol
IHC protocol for Importin Alpha 5 antibody 18137-1-AP	Download protocol
IF protocol for Importin Alpha 5 antibody 18137-1-AP	Download protocol
IP protocol for Importin Alpha 5 antibody 18137-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Nat Immunol Tumor-derived exosomes antagonize innate antiviral immunity. Authors - Liang Gao View Article
mouse	WB,IF	Acta Pharm Sin B Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. Authors - Hao Zeng View Article
human	WB	Nat Commun Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. Authors - Sergi Marco View Article
human	WB	Nat Commun CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Authors - Youzhou Sang View Article
mouse	WB	Proc Natl Acad Sci U S A Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease. Authors - Takahiro Kawasaki View Article
mouse	WB	PLoS Biol The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination. Authors - Benjamin M Laitman KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

Applications: Western Blot, Immunofluorescence
Primary Antibody Dilution: 1:1000
Cell Tissue Type: RPE-1/MSC

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Importin Alpha 5 Polyclonal antibody

Importin Alpha 5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 18137-1-AP

WB analysis of HepG2 using 18137-1-AP

IP experiment of HEK-293 using 18137-1-AP

IHC staining of human testis using 18137-1-AP

IHC staining of human testis using 18137-1-AP

IHC staining of human ovary using 18137-1-AP

IHC staining of human ovary using 18137-1-AP

IHC staining of human kidney using 18137-1-AP

IHC staining of human kidney using 18137-1-AP

IHC staining of human placenta using 18137-1-AP

IHC staining of human placenta using 18137-1-AP

IF Staining of HepG2 using 18137-1-AP

IF Staining of MCF-7 using 18137-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tumor-derived exosomes antagonize innate antiviral immunity.

Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I.

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.

The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.

Reviews