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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPNA3 Polyclonal antibody

KPNA3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28050-1-AP

Synonyms

SRP1 gamma, Qip2, Karyopherin subunit alpha-3, Karyopherin subunit alpha 3, Importin subunit alpha-4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA3 Polyclonal antibody (28050-1-AP)

    WB analysis using 28050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28050-1-AP (KPNA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brown disease using KPNA3 Polyclonal antibody (28050-1-AP)

    IHC staining of human brown disease using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KPNA3 Polyclonal antibody (28050-1-AP)

    IHC staining of mouse brain using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KPNA3 Polyclonal antibody (28050-1-AP)

    IF Staining of HepG2 using 28050-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KPNA3 antibody (28050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28050-1-AP targets KPNA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KPNA3 fusion protein Ag27755 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 3 (importin alpha 4)
Calculated molecular weight 58 kDa
Observed molecular weight57-60 kDa
GenBank accession numberBC017355
Gene symbol KPNA3
Gene ID (NCBI) 3839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA3, karyopherin subunit alpha 3, also known as SRP1 or IPOA4. It is expected to be located in cytoplasm and nucleus, the protein is ubiquitous and highly expressed in heart, skeletal muscle. The calculated molecular weight of KPNA3 is 57 kDa. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin.

Protocols

Product Specific Protocols
WB protocol for KPNA3 antibody 28050-1-APDownload protocol
IHC protocol for KPNA3 antibody 28050-1-APDownload protocol
IF protocol for KPNA3 antibody 28050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols