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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using KPNA4 Recombinant antibody (82898-2-RR)

    WB analysis of A549 using 82898-2-RR (same clone as 82898-2-PBS)

    WB result of KPNA4 antibody (82898-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells. This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KPNA4 Recombinant antibody (82898-2-RR)

    WB analysis using 82898-2-RR (same clone as 82898-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KPNA4 Recombinant antibody (82898-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82898-2-RR (same clone as 82898-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KPNA4 Recombinant antibody (82898-2-RR)

    IF Staining of U2OS using 82898-2-RR (same clone as 82898-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using KPNA4 Recombinant antibody (82898-2-RR)

    FC experiment of A549 using 82898-2-RR (same clone as 82898-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82898-2-RR

    Affinity and Kinetic Characterization of 82898-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-2-RR against Human KPNA4 were performed. The affinity constant is 0.678 nM.

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  • Featured Product
  • KD/KO Validated

KPNA4 Recombinant antibody, PBS Only

KPNA4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82898-2-PBS
Clone No.230098H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82898-2-PBS targets KPNA4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00629
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).