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KD/KO Validated

Importin Beta Polyclonal antibody

Name: Importin Beta Polyclonal antibody
Brand: Proteintech
SKU: 10077-1-AP
Rating: 4.00 (1 reviews)

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10077-1-AP

Synonyms

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10077-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse brain using 10077-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10077-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10077-1-AP (Importin beta antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of Jurkat using 10077-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (10077-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10077-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A2780 cells, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, C6 cells, HT-1080 cells, PC-12 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 15 publications below
IHC	See 2 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

10077-1-AP targets Importin Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Importin Beta fusion protein Ag0122 相同性解析による交差性が予測される生物種
Full Name	karyopherin (importin) beta 1
Calculated molecular weight	97 kDa
Observed molecular weight	90-97 kDa
GenBank accession number	BC003572
Gene symbol	KPNB1
Gene ID (NCBI)	3837
RRID	AB_2133977
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Protocols

Product Specific Protocols
WB protocol for Importin Beta antibody 10077-1-AP	Download protocol
IHC protocol for Importin Beta antibody 10077-1-AP	Download protocol
IF protocol for Importin Beta antibody 10077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development. Authors - Ping Xie View Article
	WB	Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons Authors - Carissa L Sirois View Article
human	WB	Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. Authors - Zhi-Chuan Zhu KD Validated View Article
human	WB,IP	J Transl Med TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis Authors - Xiang-Lin Hao View Article
human	WB	Virol Sin African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. Authors - Yisha Zhuo View Article
human	WB	PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication. Authors - Weiyu Luo View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100 ON). Validated with siRNA

Applications: Western Blot, Immunofluorescence
Primary Antibody Dilution: 1:1000
Cell Tissue Type: RPE-1/MSC

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis of rat brain using 10077-1-AP

WB analysis of mouse brain using 10077-1-AP

IHC staining of human colon cancer using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

FC experiment of Jurkat using 10077-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Reviews