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KD/KO Validated

KPTN Polyclonal antibody

Name: KPTN Polyclonal antibody
Brand: Proteintech
SKU: 16094-1-AP

KPTN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16094-1-AP

Synonyms

KICSTOR complex protein kaptin, Kaptin, Actin-associated protein 2E4

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 16094-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16094-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16094-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16094-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human cerebellum using 16094-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 16094-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 16094-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 16094-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16094-1-AP (KPTN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16094-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, human cerebellum tissue, mouse skeletal muscle tissue
Positive IHC detected in	human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 11 publications below

Product Information

16094-1-AP targets KPTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KPTN fusion protein Ag8785 相同性解析による交差性が予測される生物種
Full Name	kaptin (actin binding protein)
Calculated molecular weight	436 aa, 48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC009249
Gene symbol	KPTN
Gene ID (NCBI)	11133
RRID	AB_2134007
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.

Protocols

Product Specific Protocols
WB protocol for KPTN antibody 16094-1-AP	Download protocol
IHC protocol for KPTN antibody 16094-1-AP	Download protocol
IF protocol for KPTN antibody 16094-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Structure of the nutrient-sensing hub GATOR2. Authors - Max L Valenstein View Article
human	WB	Nature Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes. Authors - Kuang Shen View Article
human	WB	Nature KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1. Authors - Rachel L Wolfson KD Validated View Article
human	WB	Proc Natl Acad Sci U S A Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway Authors - Max L Valenstein View Article
human	WB	Cell Rep CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth Authors - Shasha Yin KO Validated View Article
human	WB	Cells The SZT2 Interactome Unravels New Functions of the KICSTOR Complex. Authors - Cecilia Cattelani View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPTN Polyclonal antibody

KPTN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 16094-1-AP

WB analysis using 16094-1-AP

WB analysis of HeLa using 16094-1-AP

WB analysis of NIH/3T3 using 16094-1-AP

WB analysis of human cerebellum using 16094-1-AP

WB analysis of mouse skeletal muscle using 16094-1-AP

IHC staining of human kidney using 16094-1-AP

IHC staining of human kidney using 16094-1-AP

IHC staining of human placenta using 16094-1-AP

IHC staining of human placenta using 16094-1-AP

IHC staining of human lung using 16094-1-AP

IHC staining of human lung using 16094-1-AP

IHC staining of human ovary using 16094-1-AP

IHC staining of human ovary using 16094-1-AP

IHC staining of human skin using 16094-1-AP

IHC staining of human skin using 16094-1-AP

IHC staining of human spleen using 16094-1-AP

IHC staining of human spleen using 16094-1-AP

IHC staining of human heart using 16094-1-AP

IHC staining of human heart using 16094-1-AP

IF Staining of HeLa using 16094-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Structure of the nutrient-sensing hub GATOR2.

Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.

KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.

Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.