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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KRAS-2A-specific Polyclonal antibody, PBS Only

KRAS-2A-specific Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 16156-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    WB analysis of NIH/3T3 using 16156-1-AP (same clone as 16156-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    WB analysis using 16156-1-AP (same clone as 16156-1-PBS)

    Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    IF Staining of SH-SY5Y using 16156-1-AP (same clone as 16156-1-PBS)

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KRAS-2A-specific Polyclonal antibody (16156-1-AP)

    FC experiment of HeLa using 16156-1-AP (same clone as 16156-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS-2A-specific (16156-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16156-1-PBS targets KRAS-2A-specific in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberNM_033360
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .