KRAS-2A-specific Polyclonal antibody, PBS Only

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2A-specific antibody (16156-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS-2A-specific (16156-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.